| 质粒名称: | pMK296 (DHC1-mAID Neo) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 140541 |
| 存储实验室: | Masato Kanemaki |
|
载体骨架基本信息 |
|
| 载体名称: | pCR2.1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | DHC1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMK296 (DHC1-mAID Neo) was a gift from Masato Kanemaki (Addgene plasmid # 140541 ; http://n2t.net/addgene:140541 ; RRID:Addgene_140541) |
| 参考文献部分: | The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT.. Nat Commun (2020) 11(1):5701 10.1038/s41467-020-19532-z |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pMK296 (DHC1-mAID Neo) 9177 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION DHC1 tagging with mAID.
ACCESSION .
VERSION .
KEYWORDS pMK296 (DHC1-mAID Neo)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9177)
AUTHORS Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano
R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y,
Hayashi KI, Kanemaki MT.
TITLE The auxin-inducible degron 2 technology provides sharp degradation
control in yeast, mammalian cells, and mice.
JOURNAL Nat Commun (2020) 11(1):5701
REFERENCE 2 (bases 1 to 9177)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9177
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 1314..1517
/codon_start=1
/product="minimal degron from the auxin-responsive IAA17
protein of Arabidopsis thaliana (Kubota et al., 2013)"
/label=mini-AID
/note="acts as an auxin-inducible degron (AID) in the
presence of TIR1"
/translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
AAFVKVSMDGAPYLRKIDLRMYK"
CDS 1524..2243
/codon_start=1
/product="bright green-yellow fluorescent protein derived
from mClover2, with improved photostability (Bajar et al.,
2003)"
/label=mClover3
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
primer_bind complement(1569..1590)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind 2177..2198
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 2361..2482
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2398..2417)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2452..2471
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2489..2944)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2971..3075
/gene="bla"
/label=AmpR promoter
promoter 3077..3434
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3100..3120)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3285..3420
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3347..3366
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3469..4263
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4451..4470)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
primer_bind complement(5610..5629)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5611..5629)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5636..5653)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5636..5652)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5645..5667)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 5793..6221
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS 6565..7359
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 7377..8237
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7595..7614)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8408..8996
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8897..8916
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9150..9167
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
241 gtaccttcag cgagaggatc aaacagctgc agaacatctc actggcagct gcatctggtg
301 gcgccaagga gctaaaggtg aaggcgctcc tgacgagtct cgggtggtca gcagctgtcc
361 tgggctgggg tgggagtggc tctggggaaa aacacagggc ccaggtctga cctgagctcc
421 ttcccctggg ggctgctgct ttccacagaa catccacgtg tgcctgggtg gcctgttcgt
481 gcctgaggcg tacatcactg ccaccaggca gtatgtggcc caggccaaca gctggtccct
541 ggaggagctc tgcctggaag tcaacgtcac cacctcacag ggcgccaccc ttgacgcttg
601 cagcttcgga gtcacgggtg agtggagtct cacagaaaat actggctctt tgcaggtgac
661 ctcggtggcc tgagaccatt gttcccagat acatgcactt agggtgaccg gctggcagtt
721 gggtggagcc tctgggcgcc ctgtgactgg ggtttgtgta gctgttgtgt tcacctcagc
781 ctgggttttg gcttccgcct cacaggtttg aaacttcaag gggccacgtg caacaacaac
841 aagctgtcac tgtccaatgc catctcaacc gcccttcccc tgacgcagct gcgctgggtc
901 aagcagacaa acaccgagaa gaaggccagt gtggtaagga ggcactgcct ttcccaggca
961 ttctgcaggg acccctgcgg taacaagggc agaggcggct cctcttctat gcctgggttc
1021 cacttggaac gggaaatgag gttcacagag gaaatccatt tcgcacctgt ccaaacctct
1081 ccttgccggg ccccatcagc tgtcccgggc agtcttccag ttttcttact tttcccttaa
1141 gccaccagta aacccctctg cttctgcagg taaccttacc tgtctacctg aacttcaccc
1201 gtgcagacct catcttcacc gtggacttcg aaattgctac aaaggaggac cctcgctcgt
1261 tctacgaaag aggtgtcgca gtcttgtgca cagagggatc cggtgcaggc gccaaggaga
1321 agagtgcttg tcctaaagat ccagccaaac ctccggccaa ggcacaagtt gtgggatggc
1381 caccggtgag atcataccgg aagaacgtga tggtttcctg ccaaaaatca agcggtggcc
1441 cggaggcggc ggcgttcgtg aaggtatcaa tggacggagc accgtacttg aggaaaatcg
1501 atttgaggat gtataaagct agcatggtga gcaagggcga ggagctgttc accggggtgg
1561 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg
1621 agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca
1681 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca
1741 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
1801 acgtccagga gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg
1861 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
1921 aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata
1981 tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg
2041 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
2101 ccgtgctgct gcccgacaac cactacctga gccatcagtc caagctgagc aaagacccca
2161 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg attacacatg
2221 gcatggacga gctgtacaag taatctagat aactgatcat aatcagccat accacatttg
2281 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
2341 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
2401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
2461 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
2521 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
2581 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
2641 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
2701 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
2761 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
2821 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
2881 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
2941 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
3001 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
3061 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
3121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
3181 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
3241 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
3301 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
3361 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
3421 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
3481 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
3541 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
3601 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
3661 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
3721 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
3781 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
3841 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
3901 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
3961 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
4021 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
4081 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
4141 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
4201 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
4261 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
4321 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
4381 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
4441 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
4501 taaaaagaca gaataaaacg cacggtgttg ggtcgttgga tccacttttc tagctgcccc
4561 tttctgtaat agtgaaagtt ggtatttaac atttattcat ttttaaaata tttggaaggt
4621 ctgagcttgt gaaaagaaag tggttggtct gaggttggag gaagctgaat ggaatctgac
4681 ggttgggagt ggtggaaatt ggaaggatac caggaggtat ttgggaaggc caatggcgtg
4741 gctcctttga ggaaataaaa cactaagcat gagccggctc cgcctcttct gtctccgctt
4801 tcatcccagg gcacagagcc ttgccttcca tgctgcccag ggagggcagc ccacggcagc
4861 catgcccctc cccacctcgc tttcatcatg agctcgctcc cgagcggcca cagcactcat
4921 gaatgaagac cttggggccc ttcacagaca cagatgcagc cagctgtggc tctgaaggcc
4981 ctggggcccg ggcaccatgg ttcacacctt taatcgcagc actttgggag tctgggagtt
5041 aaagaccagc ctcggcaaca tagtgagacc ccgtctctac aggaaattaa atcaggtgtg
5101 gtggtgcatg cctgtagtcc cagctacttg agggctgagg tgggaggatc acccaagccc
5161 aagaggtcga ggctgcagtg agctgtgatc tcaccactgc actccagcct gggtgacaga
5221 gcaagaccct gtctcaaaaa aaaaaagctg ggcgtggtgg ctcatgcctg taatcccagc
5281 actttgggag gccgaggcgg gcggatcacc aaattagccg ggcatggtgg cacatgcctg
5341 taatcccagc tactcgggag gctgaggcag gagaattgct tgaacctggg aggcggaggt
5401 tgcagtgagc tgaaaaaaaa aaaggcagcc cccagccgct tgtgttcttg accagggccc
5461 caggacttgg ctcctccaga caagggagtt ttgtgctgta gatgagggag ttgcccatcg
5521 ccgccctagc aagtccattc ccacacgacc tttccagtgg tgatgatgac agtggcccac
5581 tcgagcatgc atctagaggg cccaattcgc cctatagtga gtcgtattac aattcactgg
5641 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
5701 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
5761 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc
5821 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
5881 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
5941 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
6001 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
6061 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
6121 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
6181 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa ttcagggcgc
6241 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
6301 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
6361 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
6421 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
6481 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
6541 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
6601 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
6661 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
6721 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
6781 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
6841 ctattgggcg aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa
6901 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
6961 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
7021 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
7081 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
7141 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
7201 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
7261 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
7321 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga
7381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
7441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
7501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
7561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
7621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
7681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
7741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
7801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
7861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
7921 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
7981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
8041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
8101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
8161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
8221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
8281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
8341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
8401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
8461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
8521 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
8581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
8641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
8701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
8761 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
8821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
8881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
8941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
9001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
9061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
9121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
